3D tissue-like assemblies: A novel approach to investigate virus-cell interactions.
Identifieur interne : 001386 ( Main/Exploration ); précédent : 001385; suivant : 0013873D tissue-like assemblies: A novel approach to investigate virus-cell interactions.
Auteurs : Thomas J. Goodwin [États-Unis] ; Maureen Mccarthy [États-Unis] ; Randall J. Cohrs [États-Unis] ; Benedikt B. Kaufer [Allemagne]Source :
- Methods (San Diego, Calif.) [ 1095-9130 ] ; 2015.
Descripteurs français
- KwdFr :
- Bioréacteurs, Herpesviridae (physiologie), Herpèsvirus humain de type 3 (physiologie), Humains, Interactions hôte-pathogène, Poumon (virologie), Techniques de culture cellulaire, Virus du SRAS (physiologie), Virus parainfluenza humain de type 3 (physiologie), Virus respiratoires syncytiaux (physiologie).
- MESH :
English descriptors
- KwdEn :
- MESH :
- physiology : Herpesviridae, Herpesvirus 3, Human, Parainfluenza Virus 3, Human, Respiratory Syncytial Viruses, SARS Virus.
- virology : Lung.
- Bioreactors, Cell Culture Techniques, Host-Pathogen Interactions, Humans.
Abstract
Virus-host cell interactions are most commonly analyzed in cells maintained in vitro as two-dimensional tissue cultures. However, these in vitro conditions vary quite drastically from the tissues that are commonly infected in vivo. Over the years, a number of systems have been developed that allow the establishment of three-dimensional (3D) tissue structures that have properties similar to their in vivo 3D counterparts. These 3D systems have numerous applications including drug testing, maintenance of large tissue explants, monitoring migration of human lymphocytes in tissues, analysis of human organ tissue development and investigation of virus-host interactions including viral latency. Here, we describe the establishment of tissue-like assemblies for human lung and neuronal tissue that we infected with a variety of viruses including the respiratory pathogens human parainfluenza virus type 3 (PIV3), respiratory syncytial virus (RSV) and SARS corona virus (SARS-CoV) as well as the human neurotropic herpesvirus, varicella-zoster virus (VZV).
DOI: 10.1016/j.ymeth.2015.05.010
PubMed: 25986169
Affiliations:
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Goodwin</name><affiliation wicri:level="2"><nlm:affiliation>Disease Modelling/Tissue Analogues Laboratory, NASA Johnson Space Center, Houston, TX, USA. 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However, these in vitro conditions vary quite drastically from the tissues that are commonly infected in vivo. Over the years, a number of systems have been developed that allow the establishment of three-dimensional (3D) tissue structures that have properties similar to their in vivo 3D counterparts. These 3D systems have numerous applications including drug testing, maintenance of large tissue explants, monitoring migration of human lymphocytes in tissues, analysis of human organ tissue development and investigation of virus-host interactions including viral latency. Here, we describe the establishment of tissue-like assemblies for human lung and neuronal tissue that we infected with a variety of viruses including the respiratory pathogens human parainfluenza virus type 3 (PIV3), respiratory syncytial virus (RSV) and SARS corona virus (SARS-CoV) as well as the human neurotropic herpesvirus, varicella-zoster virus (VZV). </div></front></TEI><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>Berlin</li><li>Colorado</li><li>Texas</li></region><settlement><li>Berlin</li></settlement></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Goodwin, Thomas J" sort="Goodwin, Thomas J" uniqKey="Goodwin T" first="Thomas J" last="Goodwin">Thomas J. Goodwin</name></region><name sortKey="Cohrs, Randall J" sort="Cohrs, Randall J" uniqKey="Cohrs R" first="Randall J" last="Cohrs">Randall J. Cohrs</name><name sortKey="Mccarthy, Maureen" sort="Mccarthy, Maureen" uniqKey="Mccarthy M" first="Maureen" last="Mccarthy">Maureen Mccarthy</name></country><country name="Allemagne"><region name="Berlin"><name sortKey="Kaufer, Benedikt B" sort="Kaufer, Benedikt B" uniqKey="Kaufer B" first="Benedikt B" last="Kaufer">Benedikt B. Kaufer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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